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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and thus may lead to tumorigenesis. This protein was identified to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a critical position in advertising mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two more splice variants were documented but detailed nucleotide sequence data is not but accessible.
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Blocking peptides are peptides that bind specifically into the concentrate on antibody and block antibody binding. These peptide generally consists of the epitope recognized via the antibody.
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This information outlines more attainable optimization of mobile and immunoassay parameters to ensure the very best benefits are...
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This tutorial outlines additional probable optimization of mobile and immunoassay parameters to be sure the best possible benefits are...
Antibodies certain to the blocking peptide not bind for the epitope on the target protein. By comparing the staining within the blocked antibody vs . the antibody by itself, you can see which staining is unique.
Goal Facts Cyclin E belongs into the extremely conserved cyclin spouse and children, whose associates are characterised by a remarkable periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation patterns which contribute on the temporal coordination of each mitotic function. Cyclin E forms a fancy with and capabilities being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates with the G1-S stage boundary and is also degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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